Antibacterial Activity Of Lime Peel (Citrus Aurantifolia (Christm.) Swingle) Ethanolic Extract Against Skin Infection Bacteria (Propionibacterium Acnes And Staphylococcus Epidermidis)
Abstract
The skin is the outermost part of the body that serves as a protective body. In
addition to protecting the skin of the face, it also acts as a beautifier. The main
problem with facial skin is inflammation of the skin, which can cause acne.
Bacteria like Propionibacterium acnes, Staphylococcus aureus, Staphylococcus
epidermidis, and Pseudomonas aeruginosa can cause acne on the face.
Prolonged use of antibiotics as therapy against acne will cause a new problem,
namely resistance to antibiotics. Lime peel has many uses, especially as an
antibacterial, which makes it a good candidate for use as an antibacterial agent
in acne treatment. The goal of this study was to find out if the etTMhanolic extract
of lime peel could kill bacteria like Propionibacterium acnes and Staphylococcus
epidermidis. The research method used the Kirby-Bauer method and the
Streaking methods to analyze the minimum inhibitory concentration and minimum
bactericidal concentration. The results showed MIC at a concentration of 1
mg/mL for each bacterium, while MBC at a concentration of 40 mg/mL in
Propionibacterium acnes and 50 mg/mL in Staphylococcus epidermidis. The
conclusion is that the extract of lime peel in ethanol can kill bacteria.
Downloads
References
J. K. Nuzantry and R. I. Widayati, ‘Efektivitas campuran ekstrak aloe vera dan olive oil dalam formulasi pelembab pada kekeringan kulit’, PhD Thesis, Faculty of Medicine, 2015.
R. I. Tranggono and F. Latifah, ‘Buku pegangan ilmu pengetahuan kosmetik’, Jkt. PT Gramedia Pustaka Utama, vol. 3, no. 47, pp. 58–59, 2007.
R. N. Afriyanti, ‘Akne vulgaris pada remaja’, J. Major., vol. 4, no. 5, 2015.
D. R. Habibie and D. Aldo, ‘Sistem pakar untuk identifikasi jenis jerawat dengan metode certainity factor’, JOINTECS J. Inf. Technol. Comput. Sci., vol. 4, no. 3, pp. 79–86, 2019.
S. H. Pelen, ‘Formulasi Sediaan Gel Antijerawat Minyak Atsiri Kulit Batang Kayu Manis (Cinnamomum Burmanii) Dan Uji Aktivitas Terhadap Bakteri Staphylococcus Aureus’, Pharmacon, vol. 5, no. 4, 2016.
S. T. Pratiwi, ‘Mikrobiologi farmasi’, 2008.
N. Carolia and W. Noventi, ‘Potensi ekstrak daun sirih hijau (Piper betle L.) sebagai alternatif terapi Acne vulgaris’, J. Major., vol. 5, no. 1, pp. 140–145, 2016.
B. Muthmainna, ‘FORMULASI DAN UJI STABILITAS FISIK SEDIAAN GEL EKSTRAK BUAH BELIMBING WULUH (Averrhoa bilimbi L.)’, J. Pharm. Sci. Herb. Technol., vol. 4, no. 1, pp. 4–7, 2019.
A. Razak, A. Djamal, and G. Revilla, ‘Uji daya hambat air perasan buah jeruk nipis (Citrus aurantifolia s.) terhadap pertumbuhan bakteri Staphylococcus Aureus secara In Vitro’, J. Kesehat. Andalas, vol. 2, no. 1, pp. 05–08, 2013.
P. W. Parama, I. D. M. Sukrama, and S. A. Handoko, ‘Uji efektifitas antibakteri ekstrak buah jeruk nipis (Citrus aurantifolia) terhadap pertumbuhan Streptococcus mutans in vitro’, Bali Dent. J., vol. 3, no. 1, pp. 45–52, 2019.
A. F. Mutaqien, ‘ANTISEPTIK DARI JERUK NIPIS (Citrus aurantifolia (Christm.) Swingle) UNTUK MENGHAMBAT PERTUMBUHAN BAKTERI Staphylococcus aureus PADA TANGAN (Analisis Deskriptif Kualitatif dengan Teknik Studi Literatur)’, PhD Thesis, FKIP UNPAS, 2021.
T. O. Tafonao, ‘FORMULASI DAN UJI AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL KULIT JERUK NIPIS (Citrus aurantifolia (Christm.) Swingle) DALAM SEDIAAN DEODORAN TERHADAP Staphylococcus epidermidis’, PhD Thesis, Institut Kesehatan Helvetia, 2019.
S. Dalimartha and F. Adrian, Fakta Ilmiah Buah Sayur. Penebar PLUS+, 2013.
S. Dalimartha and F. Adrian, Khasiat buah dan sayur. Penebar Swadaya Grup, 2011.
A. R. Hafsari, T. Cahyanto, T. Sujarwo, and R. I. Lestari, ‘Uji aktivitas antibakteri ekstrak daun beluntas (pluchea indica (l.) less.) terhadap propionibacterium acnes penyebab jerawat’, J. Istek, vol. 9, no. 1, 2015.
R. R. Junardi, ‘PEMANFAATAN EKSTRAK KULIT JERUK PONTIANAK (Citrus nobilis L. var microcarpa) DAN EKSTRAK DAUN BELIMBING WULUH (Averrhoa bilimbi L.) DALAM PEMBUATAN HAND SANITIZER’, 2021.
N. NURDIANI, ‘Formulasi Sediaan Sabun Cair Ekstrak Etanol Kulit Jeruk Nipis (Citrus aurantifolia Swingle) Terhadap Aktivitas Antibakteri Dengan Penambahan VCO Sebagai Basis’, 2022.
R. I. Depkes, ‘Farmakope indonesia edisi IV’, Jkt. Dep. Kesehat. Repub. Indones., vol. 45, 1995.
R. I. Depkes, ‘Profil kesehatan republik indonesia’, Jkt. Badan Penelit. Dan Pengemb. Kesehat. Kemenkes RI, 2017.
N. Nasri, V. E. Kaban, H. D. Syahputra, and D. Satria, ‘Aktivitas Antibakteri Ekstrak Etanol Daun Alpukat (Persea americana Mill) Terhadap Escherichia coli, Salmonella typhi, dan Pseudomonas aeruginosa’, Herb. Med. J., vol. 5, no. 1, pp. 13–19, 2022.
N. R. Farnsworth, ‘Biological and phytochemical screening of plants’, J. Pharm. Sci., vol. 55, no. 3, pp. 225–276, 1966.
J. B. Harbone, ‘Metode Fitokimia: Penuntun Cara Modern Menganalisa Tumbuhan, Terjemah Padmawinata, K’, Dan Soediro Ed. Ii Pnb. ITB Bdg., 1987.
N. Nasri, V. E. Kaban, K. Gurning, H. D. Syahputra, and D. Satria, ‘Aktivitas Antibakteri Ekstrak Etanol Daun Pepaya (Carica papaya Linn.) Terhadap Bakteri Pseudomonas aeruginosa’, INSOLOGI J. Sains Dan Teknol., vol. 1, no. 3, Art. no. 3, Jun. 2022, doi: 10.55123/insologi.v1i3.438.
L. Waluyo, ‘Teknik Metode Dasar dalam Mikrobiologi’, UPT Pnb. Univ. Muhammadiyah Malang, 2008.
A. A. Mostafa, A. A. Al-Askar, K. S. Almaary, T. M. Dawoud, E. N. Sholkamy, and M. M. Bakri, ‘Antimicrobial activity of some plant extracts against bacterial strains causing food poisoning diseases’, Saudi J. Biol. Sci., vol. 25, no. 2, pp. 361–366, 2018.
N. Nasri, U. Harahap, J. Silalahi, and D. Satria, ‘Antibacterial activity of lactic acid bacteria isolated from Dengke Naniura of Carp (Cyprinus carpio) against diarrhea-causing pathogenic bacteria’, Biodiversitas J. Biol. Divers., vol. 22, no. 8, 2021, doi: https://doi.org/10.13057/biodiv/d220802.
D. L. Y. Handoyo and M. E. Pranoto, ‘Pengaruh Variasi Suhu Pengeringan Terhadap Pembuatan Simplisia Daun Mimba (Azadirachta Indica)’, J. Farm. Tinctura, vol. 1, no. 2, pp. 45–54, 2020.
V. J. Anggraeni, T. S. Wahyu, H. Kusriani, and D. Kurnia, ‘Aktivitas Antibakteri Ekstrak Mikroalga Thalassiosira Sp Terhadap Bakteri Staphylococcus Aureus, Staphylococcus Epidermidis Dan Propionibacterium Acne’, J. Kim. Ris., vol. 4, no. 1, pp. 62–73, 2019.
M. Meliyana and R. Ridwanto, ‘Uji Aktivitas Antioksidan Ekstrak Etanol Daun Jeruk Kasturi (Citrus microcarpa Bunge) Di Daerah Labuhanbatu, Sumatera Utara Dengan Metode DPPH (1, 1Diphenyl-2-Picrylhydrazil)’, J. Health Med. Sci., pp. 100–109, 2022.
S. Supartiningsih, J. K. Marpaung, A. Adiansyah, and H. J. Siregar, ‘UJI AKTIVITAS ANTIDIABETES EKSTRAK ETANOL DAUN SENDOK (Plantago major L.) PADA TIKUS PUTIH JANTAN YANG DIINDUKSI ALOKSAN’, J. FARMANESIA, vol. 5, no. 1, pp. 46–50, 2018.
F. A. Rahman, T. Haniastuti, and T. W. Utami, ‘Skrining fitokimia dan aktivitas antibakteri ekstrak etanol daun sirsak (Annona muricata L.) pada Streptococcus mutans ATCC 35668’, Maj. Kedokt. Gigi Indones., vol. 3, no. 1, pp. 1–7, 2017.
W. W. Davis and T. R. Stout, ‘Disc plate method of microbiological antibiotic assay: II. Novel procedure offering improved accuracy’, Appl. Microbiol., vol. 22, no. 4, pp. 666–670, 1971.
M. E. Hapsari, ‘Uji Aktivitas Antibakteri Ekstrak Herba Meniran (Phyllanthus niruri) Terhadap Pertumbuhan Bakteri Bacillus cereus Dan Escherichia coli’, Skripsi Univ. Sanata Dharma Yogyak. Hal, vol. 8, 2015.
M. Balouiri, M. Sadiki, and S. K. Ibnsouda, ‘Methods for in vitro evaluating antimicrobial activity: A review’, J. Pharm. Anal., vol. 6, no. 2, pp. 71–79, 2016.
W. Astriyani, P. Surjowardojo, and T. E. Susilorini, ‘Daya Hambat Ekstrak Buah Mahkota Dewa (Phaleria macrocarpa L.) Dengan Pelarut Ethanol dan Aquades Terhadap Bakteri Staphylococcus Aureus Penyebab Mastitis pada Sapi Perah’, TERNAK Trop. J. Trop. Anim. Prod., vol. 18, no. 2, pp. 8–13, 2017.
A. Luthfiyani and P. Pujiastuti, ‘Daya Antibakteri Ekstrak Daun Seledri (Apium graveolens L.) terhadap Porphyromonas gingivalis’, STOMATOGNATIC-J. Kedokt. Gigi, vol. 16, no. 2, pp. 53–58, 2019.
N. M. Atef, S. M. Shanab, S. I. Negm, and Y. A. Abbas, ‘Evaluation of antimicrobial activity of some plant extracts against antibiotic susceptible and resistant bacterial strains causing wound infection’, Bull. Natl. Res. Cent., vol. 43, no. 1, pp. 1–11, 2019.
A. R. War, A. A. Buhroo, B. Hussain, T. Ahmad, R. M. Nair, and H. C. Sharma, ‘Plant defense and insect adaptation with reference to secondary metabolites’, in Co-Evolution of Secondary Metabolites, Springer, 2020, pp. 795–822.
B. Prajwala, M. B. Kanthesh, and T. S. Gopenath, ‘Studies on Inhibitory Potentials of Tinospora Cordifolia Plant Extract against Pathogenic Bacteria’, Greens Trust, 2021.
M. Muhammad, N. Nasri, V. E. Kaban, D. Satria, and H. Cintya, ‘Antibacterial Potential Ethanol Extract of Papaya Leaves (Carica papaya Linn.) Towards Salmonella typhi’, BEST J. Biol. Educ. Sains Technol., vol. 5, no. 2, pp. 265–270, 2022.
M. F. Lubis, V. E. Kaban, J. O. Aritonang, D. Satria, A. A. Mulina, and H. Febriani, ‘ACUTE TOXICITY AND ANTIFUNGAL ACTIVITY OF THE OINTMENT Murraya koenigii ETHANOL EXTRACT’.