Isolation of Chitosan From Dogol Shrimp Skin (Parapenaeopsis sculptilis)

  • Dea Anggreini Postgraduate Programs, Faculty of Pharmacy, Universitas Sumatera Utara
  • Yuandani Yuandani Department of Pharmacology, Faculty of Pharmacy, Universitas Sumatera Utara, Jl. Tri Dharma No. 5, Kampus USU, Medan, Sumatera Utara, Indonesia 20155
  • Siti Morin Sinaga Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Sumatera Utara, Jl. Tri Dharma No. 5, Kampus USU, Medan, Sumatera Utara, Indonesia 20155
Keywords: Chitosan, shrimp shell, characterization, FT-IR


In Indonesia, shrimp underwent a "cold storage" process where the heads, tails, and shells were discarded as waste. This shrimp waste could pollute the environment around the factory so it needed to be utilized. So far, shrimp shells had only been used as ingredients for making crackers, shrimp paste, and animal feed supplements. The remaining shrimp shells that had been separated were made into chitosan which was then subjected to characterization tests. The three steps of the chitosan isolation process were deproteinization, demineralization, and deacetylation. The analytical tests carried out were testing the water content, ash content, and functional groups contained in chitosan which were identified using the Fourier Transform Infra Red (FT-IR) Spectrophotometer. Based on the test results, there was a moisture content of 9.27% ​​and an ash content of 1.69% in the chitosan samples made from shrimp shells. Functional groups of shrimp shell chitosan were identified using FT-IR characteristics. The -NH- group, which was represented by the N-H and O-H stretching groups, could be seen in the absorption band 1456.26 in the wave range 3000–30500. The conclusion of this study was that chitosan could be extracted from shrimp shells and meet the characterization criteria.


Download data is not yet available.


[1] I. Goligo, ‘Subsektor Perikanan’, Makasar: Bone, 2009.
[2] K. Haryani, H. Hargono, and C. S. Budiyati, ‘Pembuatan Khitosan dari Kulit Udang untuk Mengadsorbsi Logam Krom (Cr6+) dan Tembaga (Cu)’, Reaktor, vol. 11, no. 2, pp. 86–90, 2007.
[3] R. S. Sulistiyoningrum, J. Suprijanto, and A. Sabdono, ‘Aktivitas anti bakteri kitosan dari cangkang kerang simping pada kondisi lingkungan yang berbeda: kajian pemanfaatan limbah kerang simping (Amusium sp.)’, Journal of marine research, vol. 2, no. 4, pp. 111–117, 2013.
[4] H. Celebi and A. Kurt, ‘Effects of processing on the properties of chitosan/cellulose nanocrystal films’, Carbohydrate polymers, vol. 133, pp. 284–293, 2015.
[5] Z. D. Nurfarjin, ‘Karakterisasi dan sifat biodegradasi edible film dari pati kulit pisang nangka (musa paradisiaca l.) dengan penambahan kitosan dan plasticizer gliserol’, in Seminar Nasional Teknik Kimia Kejuangan, 2015, pp. 1-1-L1. 7.
[6] B. V. Gaikwad, J. M. Koli, and A. S. Desai, ‘Isolation and characterization of chitosan from crab (Scylla serrata) shell waste’, Int. J. Sci. Appl. Res, vol. 2, no. 6, 2015.
[7] A. M. Silalahi, A. Fadholah, and L. O. Artanti, ‘Isolasi dan Identifikasi Kitin Dan Kitosan Dari Cangkang Susuh Kura (Sulcospira testudinaria)’, Pharmaceutical Journal of Islamic Pharmacy, vol. 4, no. 1, pp. 1–9, 2020.
[8] M. F. Lubis, P. A. Z. Hasibuan, H. Syahputra, J. M. Keliat, V. E. Kaban, and R. Astyka, ‘Duku (Lansium domesticum) Leaves Extract Induces Cell Cycle Arrest and Apoptosis of HepG2 Cells via PI3K/Akt Pathways’, Trends in Sciences, vol. 20, no. 2, pp. 6437–6437, 2023.
[9] D. I. Sánchez-Machado, J. López-Cervantes, M. A. Correa-Murrieta, R. G. Sánchez-Duarte, P. Cruz-Flores, and G. S. de la Mora-López, ‘Chitosan’, in Nonvitamin and nonmineral nutritional supplements, Elsevier, 2019, pp. 485–493.
[10] K. Gurning, ‘Characterization and Screening of Phytochemical Secondary Metabolite of Seri (Muntingia calabura, L) Leaves which is Potential as an Anti-Diabetic based on Indonesian Herbal Medicine Standard’, Journal of Drug Delivery and Therapeutics, vol. 10, no. 6-s, pp. 92–94, 2020.
[11] M. Pakizeh, A. Moradi, and T. Ghassemi, ‘Chemical extraction and modification of chitin and chitosan from shrimp shells’, European Polymer Journal, vol. 159, p. 110709, 2021.
[12] A. Dalimunthe, D. Pertiwi, M. Muhmmad, V. E. Kaban, N. Nasri, and D. Satria, ‘The effect of extraction methods towards antioxidant activity of ethanol extract of Picria fel-terrae Lour. Herbs’, in IOP Conference Series: Earth and Environmental Science, 2022, vol. 1115, no. 1, p. 012040.
[13] S. Agustina, I. M. D. Swantara, and I. N. Suartha, ‘Isolasi kitin, karakterisasi, dan sintesis kitosan dari kulit udang’, Jurnal Kimia, vol. 9, no. 2, pp. 271–278, 2015.
[14] E. Cahyono, ‘Karakteristik kitosan dari limbah cangkang udang windu (Panaeus monodon)’, Akuatika Indonesia, vol. 3, no. 2, pp. 96–102, 2018.
How to Cite
Anggreini, D., Yuandani, Y., & Sinaga, S. M. (2023). Isolation of Chitosan From Dogol Shrimp Skin (Parapenaeopsis sculptilis). International Journal of Science, Technology & Management, 4(1), 69-72.