Phytochemical Screening And Antibacterial Activity Of The Ethanol Extract Of Putri Malu (Mimosa Pudica Linn.) Leaves Combined With Klanceng Honey Against Staphylococcus Aureus Bacteria
Abstract
Mimosa pudica Linn. Leave and honey have been known to have
antibacterial activity. The combination of the two is very beneficial as an
antibacterial because the hydrogen peroxide content in honey and the
flavonoid metabolite compounds in Putri Malu provide a synergistic
effect as an antibacterial. The aim of this research was to determine the
activity of the ethanol extract of Putri Malu leaves combined with
Klanceng honey using the disc diffusion method against Staphylococcus
aureus bacteria. The results showed that the resulting extract was thick
and blackish-green in colour, with a yield of 25.6%. The results of the
phytochemical screening test showed that Putri Malu extract contains
alkaloids, flavonoids, glycosides, saponins, and tannins. Characterization
results showed that the water content of the extract was 7.28 ± 0.05,
water-soluble essence content was 16.03 ± 0.08, ethanol-soluble essence
content was 19.65 ± 0.01, total ash content was 4.70 ± 0.00, and essence
content acid soluble was 0.17±0.02. Antibacterial testing showed that the
best ratio of Putri Malu leaf extract to honey to inhibit Staphylococcus
aureus was 9:1, with an inhibitory value of 13.60 ± 0.40 mm. In
conclusion, the combination of Putri Malu leaf extract with honey
strongly inhibits the growth of Staphylococcus aureus bacteria.
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