Simultaneous Analysis of Tablets Content Vitamin C And Zinc In Visible Spectrophotometry

  • Sulasmi Sulasmi Postgraduate Programs, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, Indonesia
  • Muchlisyam Muchlisyam Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, Indonesia
  • Ginda Haro Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, Indonesia
Keywords: Simultaneous Analysis, Vitamin C, Zinc and Spectrophotometry Visible.

Abstract

Most drugs on the market contain multiple active ingredients, each of which is intended to enhance the drug's therapeutic effect and ease of administration. Determination of the levels of efficacious substances in drug preparations is essential for agencies that conduct drug determinations, such as the Food and Drug Supervisory Agency (BPOM) and the drug industry; therefore, a rapid and dependable analytical method, as well as relatively inexpensive and easily accessible tools and operational costs, are required. In practice, however, it can produce accurate and precise results. This research will be conducted by optimizing the solvent and simultaneously analyzing the levels of Vitamin C and Zinc in the tablet preparation without any separation step using visible spectrophotometry. Vitamin C and Zinc were found to be soluble in a mixture of methanol and water with a ratio of 50:50 after a single examination with the addition of the complexing agent dhitizon at a concentration of 0.1%, which produced a pink color. Calculation of Vitamin C and Zinc's linearity. The linearity value described is the correlation coefficient value for both Vitamin C and Zinc, which is very close to one, indicating an excellent relationship between the drug concentration and the absorbance value. This also suggests that absorbance will increase as concentration increases. The method validation requirements for linearity, accuracy, and precision as well as the limit of detection (LOD) and the limit of quantification (LOQ) were met by the analytical method validation with promising results.

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References

Mitmesser, S.H., Ye, Q., Evans, M., Combs, M., (2016). Determination of plasma and leukocyte Vitamin C

concentrations in a randomized, double-blind, placebo-controlled trial with Ester-C®. Springer Plus 5.

doi : 10.1186/s40064-016-2605-7.

Prasad AS (1998) Seng dan kekebalan. Biokimia Sel Mol 188: 63-69.

Bhowmik, D. Chiranjib, K.P. Kumar, S. (2010). A Potential Medicinal Importance of Zink in Human Health

and Chronic Disease. Department of Pharmaceutical Sciences, Coimbatore Medical College, Coimbatore,

Tamilnadu, India Int J Pharm Biomed Sci ;1(1), 05-11.

Naid, T.,Kasim S. dan Pakaya, M.(2011). Penetapan Kadar Parasetamol dalam Tablet Kombinasi Parasetamol

dengan Kafein secara Spektrofotometri Ultraviolet sinar tampak. Majalah Farmasi dan Farmakologi, Vol 15 :

- 82.

International Journal of Science, Technology & Management ISSN: 2722 - 4015

http://ijstm.inarah.co.id

Khoshayand, M.R., Abdollahi, H., Ghaffari, A., Shariatpanahi, M., dan Farzanegan, H. (2010). Simultaneous

spectrophotometric determination of paracetamol, phenylephrine and chlropheniramine in pharmaceuticals using

chemometric approaches. Daru journal of pharmaceutical sciences, 18(4): 292–297.

Hajian, R., danAfshari, N. (2012).The Spectrophotometric Multicomponent Analysis of a Ternary Mixture of

Ibuprofen, Caffeine and Paracetamol by the Combination of Double Divisor-Ratio Spectra Derivative and HPoint Standard Addition Method.E-Journal of Chemistry, 9(3): 1153–1164.

Zareba S dan Pomykalski A, 2003). Spectrophotometric determination of Zn in pharmaceutical preparations:

Antioxidant and Zinkuprin Forte by azodyes derivatives of thriazolo diazophenols. Vol 56(6) : 94-101

Elgailani I.E.H danAlghamdi R.H. (2017). Determination of Vitamin C in some Pharmaceutical Dosage by UVVisible Spectrophotometer Using Bromocresol Purple as a Chromogenic Reagent. Der PharmaChemica,Vol

(16):28-32.

Harmita. (2004). Petunjuk Pelaksanaan Validasi Metode dan Cara Perhitungannya. Majalah Ilmu Kefarmasian,

I(3), 117–135.

Gandhimathi, R., Vijayaraj, S., dan Jyothirmaie, M.P. (2012). Analytical Process of Drugs By Ultraviolet (UV)

Spectroscopy-A Review. International Journal of Pharmaceutical Researchdan Analysis, 2(2): 72–78.

Zulnaidi. 2007. Metode Penelitian. [Karya Ilmiah]. Fakultas Sastra, Universitas Sumatra Utara, Medan, hlm. 17

Departemen Kesehatan RI. (1995). Farmakope Indonesia Edisi IV. Jakarta : Departemen Kesehatan RI. 1033.

Ham, B.M., Maham, A. (2016). Analytical Chemistry. Canada: John

Wiley & Sons, Inc.

Harahap, A., et all ( 2021), Monitoring Of Macroinvertebrates Along Streams Of Bilah River International

Journal of Conservation Sciencethis link is disabled, 12(1), pp. 247–258.

Mamangkey, J., Suryanto, D., et all (2021). Isolation and enzyme bioprospection of bacteria associated to

Bruguiera cylindrica, a mangrove plant of North Sumatra, Indonesia, Biotechnology Reports, 2021, 30, e00617.

Christian, Gary D., 1994, Analytical Chemistry, edisi ke-5, John Wiley & Sons Inc., New York.

Harmita. (2004). Petunjuk Pelaksanaan Validasi Metode dan Cara Perhitungannya. Majalah Ilmu Kefarmasian,

I(3), 117–135.

Harmita, H. 2012. Petunjuk pelaksanaan validasi smetode dan Cara Perhitungannya. Pharmaceutical Sciences

and Research (PSR), 1(3): 117–135

Published
2023-03-19
How to Cite
Sulasmi, S., Muchlisyam, M., & Haro, G. (2023). Simultaneous Analysis of Tablets Content Vitamin C And Zinc In Visible Spectrophotometry. International Journal of Science, Technology & Management, 4(2), 386-392. https://doi.org/10.46729/ijstm.v4i2.782
Section
Articles