Rhizobacteria as Biological Agents in Enhancing the Viability and Vigor of Rice Seeds (Oryza sativa L.)

I  Kadek Pande Prasetia Wiguna; Gusti Ayu Kade Sutariati; Tresjia  Corina Rakian

doi:10.46729/ijstm.v6i1.1263

I Kadek Pande Prasetia Wiguna Master's student in Agronomy, Faculty of Agriculture, Halu Oleo University, Kendari, 93232, Indonesia
Gusti Ayu Kade Sutariati Department of Agrotechnology, Agriculture Faculty, Halu Oleo University, Kendari 93232, Indonesia
Tresjia Corina Rakian Department of Agrotechnology, Agriculture Faculty, Halu Oleo University, Kendari 93232, Indonesia

DOI: https://doi.org/10.46729/ijstm.v6i1.1263

Keywords: Rhizobacteria, seed viability, vigor index and biofertilizer.

Abstract

This study aims to examine the effectiveness of rhizobacteria in stimulating plant growth and acting as a biofertilizer that can enhance the viability and vigor of rice (Oryza sativa L.) seeds. The research was conducted at the Agronomy Unit Laboratory, Faculty of Agriculture, Halu Oleo University, from September to October 2024. The study was designed based on a randomized block design (RBD), consisting of 11 test isolates and 1 control. These isolates were tested for their effectiveness in improving the viability and vigor of rice (Oryza sativa L.). The test was carried out by soaking seeds in a rhizobacteria suspension, then germinating the seeds using a standard germination procedure. The observed variables included germination capacity, vigor index, seed uniformity, relative growth rate, and T50. The results showed that seed treatment with rhizobacteria significantly increased the viability and vigor of rice seeds (Oryza sativa L.), as seen in the variables of germination capacity, vigor index, T50, and relative growth rate. From this study, 2 isolates were selected that have the potential as growth promoters for rice seeds, namely DMW 4 and DSS 3. Further research is needed to test the effectiveness of these isolates in improving the growth and yield of rice (Oryza sativa L.) under field conditions.

Downloads

Download data is not yet available.

References

Ashrafuzzaman, M., Hossen, F.H., Ismail, M.R., Hoque, M.A., Islam, M.Z., Shahidullah, S.M. and Meon, S. Efficiency of Plant Growth-Promoting Rhizobacteria (PGPR) for the Enhancement of Rice Growth. African Journal of Biotechnology, 8(7), 2009, 1247–1252.

Badan Pusat Statistik [BPS]. Sulawesi Tenggara dalam Angka 2021-2023. BPS Indonesia, 2024.

Chiappero, J., Cappellari, L.R., Alderete, L.G.S., Palermo, T.B., Banchio, E. Plant growth promoting rhizobacteria improve the antioxidant status in Mentha piperita grown under drought stress leading to an enhancement of plant growth and total phenolic content. Industrial Crops & Products, 139, 2019, https://doi.org/10.1016/j.indcrop.2019.111553.

Chieb, M., and Gachomo, E.W. The Role of Plant Growth Promoting Rhizobacteria in Plant Drought Stress Responses. Plant Biology, 23, 2023, 407. https://doi.org/10.1186/s12870-023-04403-8.

Copeland, L.O., McDonald, M.B. Principles of Seed Science and Technology. Third Edition. New York: Chapmond & Hall 1995.

Elango, R., Parthasarathi, R., Megala, S. Field level studies on the association of plant growth promoting rhizobacteria (PGPR) in Gloriosa Superba L, rhizosphere. Indian Streams Research Journal, 3(10), 2013, 1-6.

El‑Mageed, T.A.A., El‑Mageed, S.A.A., El‑Saadony, M.T., Abdelaziz, S., and Abdou, M.N. Plant Growth‑Promoting Rhizobacteria Improve Growth, Morph‑Physiological Responses, Water Productivity, and Yield of Rice Plants Under Full and Deficit Drip Irrigation. Research Rice. 2022. https://doi.org/10.1186/s12284‑022‑00564‑6.

Jiang, Y., Wu, Y., Xu, W., Cheng, Y., Chen, J., Xu, L., Hu, F., Li, H. IAA-producing bacteria and bacterial-feeding nematodes promote Arabidopsis thaliana root growth in natural soil. European Journal of Soil Biology, 52, 2012, 20-26.

Lata, R. dan Gond, S. Plant growth-promoting microbes for abiotic stress tolerance in plants. In: Role of Plant Growth Promoting Microorganisms in Sustainable Agriculture and Nanotechnology book, 2019. 89-105. DOI:10.1016/B978-0-12-817004-5.00006-3.

Leite, M.S, Pinto, T.E.F., Centofante, A.R., Neto, A.R., Silva, F.G., Selari, P.J.R.G., Martins, P.F. Acclimatization of Pouteria gardeneriana Radlk micropropagated plantlets: Role of in vitro rooting and plant growth–promoting bacteria. Current Plant Biology, 27, 2021, DOI:10.1016/j.cpb.2021.100209.

Mardiah, Syamsuddin, dan Efendi. Perlakuan Benih Menggunakan Rizobakteri Pemacu Pertumbuhan Terhadap Pertumbuhan Vegetatif Dan Hasil Tanaman Cabai Merah (Capsicum annuum L.). J. Floratek, 11(1), 2016, 25-35

Marom, N., Rizal, F.N.U. dan Bintoro, M. Uji Efektivitas Saat Pemberian dan Konsentrasi PGPR (Plant Growth Promoting Rhizobacteria) terhadap Produksi dan Mutu Benih Kacang Tanah (Arachis hypogaea L.). Journal of Applied Agricultural Sciences, 1(2), 2017, 174–184.

Mudi, L. dan Sutariati, G.A.K. Aplikasi Konsorsium Endo-Rhizobakteri untuk Meningkatkan Vigor Benih Padi Gogo Lokal. Jurnal Agrotech, 11(1), 2021, 1–7.

Nasution, H.F. Budidaya Padi yang Paling Menguntungkan. Garuda Pustaka. Jakarta Timur. 2019.

Pranata, A.D. Meningkatkan Hasil Panen dengan Pupuk Organik. PT Agomedia Pustaka Jakarta. 2010.

Putrie, R.F. Plant Growth Promoting Rhizobacteria (PGPR) Penghasil Eksopoliosakarida Sebagai Inokulan Area Pertanian Lahan Kering. Biotrends, 7(1), 2016, 35-41.

Sadjad, S., Murniati, E., Ilyas, S. Parameter Pengujian Vigor Benih dari Komparatif ke Simulatif. Jakarta: Grasindo 1999 [Indonesian].

Sadjad, S. Kekuatan Tumbuh Benih. Penataran Penyuluhan Pertanian Spesialis. Bagian Penataran BIMAS. Departemen Agronomi IPB. Bogor. 1972.

Santoso, U., dan Nursandi, F. Kultur Jaringan Tanaman. Malang: UMMPress. 2003.

Sari, W. dan Faisal, M.H. Pengaruh Media Penyimpanan Benih terhadap Viabilitas dan Vigor Benih Padi Pandanwangi. Agroscience, 7(2), 2017, 300–310

Sherpa, M.T., Bag, N., Das, S., Haokip, P., Sharma, L. Isolation And Characterization Of Plant Growth Promoting Rhizobacteria Isolated From Organically Grown High Yielding Pole Type Native Pea (Pisum sativum L.) variety Dentami of Sikkim, India. Current Research in Microbial Sciences, 2, 2021, DOI:10.1016/j.crmicr.2021.100068

Sun, Q., Liu, Y., Liu, H., and Dumroese, R.K. Interaction of Biochar Type and Rhizobia Inoculation Increases the Growth and Biological Nitrogen Fixation of Robinia pseudoacacia Seedlings. Forests, 11(6), 2020, 711

Sutariati, G.A.K., Rakian, T.J., Agustina, Sopacua L, Mudi L dan Haq M. Kajian Potensi Rizobakteri Pemacu Pertumbuhan Tanaman Yang Diisolasi Dari Rizosfer Padi Sehat. Jurnal Agroteknos. 4(2), 2014, 71-77.

Sutariati, G.A.K., Widodo., Sudarsono., Ilyas, S. Pengaruh Perlakuan Rhizobakteri Pemacu Pertumbuhan Tanaman terhadap Viabilitas Benih serta Pertumbuhan Bibit Tanaman Cabai. Bul. Agron, 34(1), 2006. 46-54.

Sutariati, G.A.K. Conditioning benih dengan rizobakteri untuk meningkatkan mutu fisiologis dan patologis benih cabai pratanam. Warta-Wiptek, 17, 2009, 7-16.

Syamsuddin., Marlina., Hasanuddin., Ulim, M.A. Perlakuan Rizobakteri Pemacu Pertumbuhan Tanaman (RPPT) terhadap Viabilitas dan Vigor Benih Serta Pertumbuhan Bibit Tanaman Dua Verietas Cabai Merah (Capsicum annum L,). Prosiding Seminar Nasional Biotik. 2015:382-389.

Wahyuni, S., Mulsanti, I.W., Satoto. Produktivitas Varietas Padi dari Kelas Benih Berbeda. Iptek Tanaman Pangan, 8(22), 2013.

Wibowo, T.D. Pengaruh tipe persilangan terhadap mutu fisiologis benih jagung (Zea mays L.) hibrida. Tesis. Institut Pertanian Bogor. Bogor. 2013.

Widiarti, W., Wulandari, E., and Rahardjo, P. Respons vigor benih dan pertumbuhan awal tanaman tomat terhadap konsentrasi dan lama perendaman asam klorida (HCl). Agritrop : Jurnal Ilmu-Ilmu Pertanian,14(2), 2017, 151–160. doi: 10.32528/agr.v14i2.429.