Phytochemical Screening And Antidiabetic Test Of Ethanol Extract Of Turmeric Leaves (Curcuma domestica Val.) On Decreasing Blood Glucose Of Diabetic Rats
Abstract
The turmeric plant (Curcuma domestica Val.) is a tropical plant that grows a lot on the Asian continent with is use as a food coloring and fragrance. The use of turmeric leaves by residents is only a cooking ingredient not used in large quantities. Even most of these turmeric leaves are considered waste and have minimal utilization. Turmeric leaf methanol extract can be used to lower blood glucose. Turmeric leaf extract has the potential as a valuable food source of its antioxidant components, such as total phenolic compounds and flavonoids which increase radical scavenging activity. In particular, the water extract of turmeric leaves contains high total phenolic compounds (2.741 ± 0.099 mg GAE/g) and flavonoids (4.776 ± 0.010 mg QCE/g). Diabetes mellitus is a metabolic disorder associated with several chronic complications, such as nephropathy, neuropathy, retinopathy and cardiomyopathy. Part of the turmeric plant leaves is reported to have the ability as an antioxidant. Antioxidant compounds can control blood glucose levels and prevent diabetes complications. This study was conducted to test the ability of turmeric leaf ethanol extract (EEDK) to reduce blood glucose levels in diabetic rats induced by nicotinamide and streptozotocin and to perform phytochemical screening to determine the compounds contained in turmeric leaves so that it becomes an alternative as a diabetes treatment and can prevent damage. or diabetic complications. The results showed that turmeric leaves contained secondary metabolites, namely flavonoids, glycosides, tannins, and triterpenoids/steroids, but there were no alkaloids or saponins. The characterization results show that the sample meets the requirements. Turmeric leaf ethanol extract doses of 400 mg/kg, 200 mg/kg, and 100 mg/kg could reduce the Blood Glucose Level of NA and STZ-induced rats from the fourth day of treatment.
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Kuzuya, T., Nakagawa, J.S., Kanazawa, Y., Yasuhito, I., Masachi, K., Kisihio, N., et al. (2002). Diabetes
Research and Clinical Practice. Journal of the International Diabetes Federation. Vol.55(1): 65-85.
Maulita, A. P., Susanti, S., & Setiani, B. E. (2017). Pengaruh Marinasi Ekstrak Daun Kunyit (Curcuma
domestica Val.) terhadap Kadar Air, Nilai pH, Kadar Lemak dan Kadar Protein Daging Itik. Doctoral
dissertation, Fakultas Peternakan Dan Pertanian Undip.
Hasan. (2014). Preliminary Phytochemical Screening, Toxicity, Antihyperglycemic And Analgesic Activity
Studies With Curcuma Longa Leaves. World Journal Of Pharmacy And Pharmaceutical Sciences. Volume 3,
Issue 9, 81-91.
Kim, S., Ko, S. C., Kim, Y. S., Ha, S. K., Park, H. Y., Park, Y., & Lee, S. H. (2019). Determination of Curcuma
longa L. (Turmeric) leaf extraction conditions using response surface methodology to optimize extraction yield
and antioxidant content. Journal of Food Quality, 2019.
Suryanto, E. (2019). Aktivitas Penangkal Radikal Bebas Dan Penstabil Oksigen Singlet Dari Ekstrak Daun
Kunyit (Curcuma Domestica Val.). CHEMISTRY PROGRESS, 2(2).
Brahmachari, G. (2011). Bio-flavonoids with promising antidiabetic potentials: A critical survey. Research
signpost, 661(2), 187-212.
Yan, S. W., & Asmah, R. (2010). Comparison of total phenolic contents and antioxidant activities of turmeric
leaf, pandan leaf and torch ginger flower. International Food Research Journal, 17(2), 417-423.
Widowati, W. (2008). Potensi antioksidan sebagai antidiabetes. Maranatha Journal of Medicine and Health,
(2), 149640.
Hajiaghaalipour, F., Khalilpourfarshbafi, M., & Arya, A. (2015). Modulation of glucose transporter protein by
dietary flavonoids in type 2 diabetes mellitus. International journal of biological sciences, 11(5), 508.
International Journal of Science, Technology & Management ISSN: 2722 - 4015
Sumardika, I., Jawi, I. (2012). Ekstrak Air Daun Ubi Ungu Memperbaiki Profil Lipid dan Meningkatkan Kadar
SOD Serum Darah Tikus yang Diberi Makanan Tinngi Kolesterol. Medicina. 43(2): 67-70.
Yang, R., Wang, W.X., Chen, H.J., He, Z.C. (2018). The inhibition of advanced glycation end-products by five
fractions and three main flavonoids from Camellia nitidissima Chi flowers. Journal of food and drug analysis.
: 252-259.
Departemen Kesehatan RI, (1979). Farmakope Indonesia Edisi III, 378, 535, 612.Jakarta.
Masiello, P., Broca, C., Gross, R., Roye, M., Manteghetti, M., Hillaire, B.D., et al. (1998). Experimental
NIDDM: Development of a New Model in Adult Rats Administered Streptozotocin and Nicotinamide. Diabetes.
Vol 47(2):224.
Szkudelski, T. (2012). Streptozotocin-Nicotinamide Induced Diabetes in the Rat. Characteristics of the
Experimental Model. Experimental Biology and Medicine. Vol. 237(5): 481.
Krishnasamy, G., Muthusamy, K., Chellapan, R.D. and Subbiah, N. (2016). Antidiabetic, Antihyperlipidaemic,
and Antioxidant Activity of Syzygium Densiflorum Fruits in Streptozotocin and Nicotinamide-Induced Diabetic
Rats. Pharmaceutical Biology. 54(9): 1716-1726.
World Health Organization. (1992). Quality Control Methods for Medicinal Plant Material. Switzerland:
Geneva. Halaman 26-27, 31-33.
Depkes RI. (1995). Materia Medika Indonesia. Jilid VI. Jakarta: Departemen Kesehatan Republik Indonesia.
Hal. 323-325.
Harborne, J.B. (1987). Metode Fitokimia. Penerjemah: Kosasih Padmawinata dan Iwang Soediro. Bandung:
Penerbit ITB. Hal. 6-7, 70-72, 74, 102.
Velazquez, A.L.L., Beltran, M.M., Panduro, A., Ruiz, L.H. (2011). Alternative Medicine and Molecular
Mechanisms in Chronic Degenerative Diseases. Chinese Medicine. 2: 84-92.
Goud, B.J., Dwarakanath, V., dan Swamy, B.K. (2015). Streptozotocin-A Diabetogenic Agent in Animal
Models. Human Journals. 3(1): 253-269.
Vinayagam, R., Baojun, X. (2015). Antidiabetic properties of dietary flavonoids: a cellular mechanism review.
Nutrition & Metabolism. 12(60): 1-20.
Kumari, M. and Jain S. (2012). Tannins: An Antinutrient with Positive Effect to Manage Diabetes. Research
Journal of Recent Sciences. 1(12): 1-8.
Peniati, E., Setiadi, E., Susanti, R., Iswari, R. (2017). Anti-hyperglycemic effect of Aloe vera peel extract on
blood sugar level of alloxan-induced Wistar rats. IOP Publishing. 1-5.