The Effectiveness Of Pre-Planting Seeds Bio-Matriconditioning And Application Of Fertilization With Leisa Technique On Growth Of Areca Nut Seedling (Areca Catechu L.)

  • Gusti Ayu Kade Sutariati Department of Agrotechnology, Faculty of Agriculture, University of Halu Oleo, Kendari Southeast Sulawesi 93232 Indonesia
  • Muhidin Department of Agrotechnology, Faculty of Agriculture, University of Halu Oleo, Kendari Southeast Sulawesi 93232 Indonesia
  • Nini Mila Rahni Department of Agrotechnology, Faculty of Agriculture, University of Halu Oleo, Kendari Southeast Sulawesi 93232 Indonesia
  • Gusti Ngurah Adi Wibawa Department of Statistics, Math and Science Faculty, University of Halu Oleo, Kendari Southeast Sulawesi 93232 Indonesia
  • La Mudi Department of Plantation Crop Cultivation, State Agricultural Polytechnic of Samarinda, 7513, Indonesia
Keywords: Areca nut; biomatricondtioning; inorganic fertilizer; LEISA; organic plus; seedling


Areca nut is widely used as industrial raw material, both for health and cosmetics and as a source of renewable
energy. This study aimed to examine the combination of seed bombatriconditioning and LEISA fertilization
treatments that were effective in increasing the growth of areca nut seedlings. The research was carried out in the
Wua-Wua Kendari Village, from March to July 2021. The study used a split-plot design with a completely
randomized design (CRD). The main plot is seed biomatriconditioning treatment which consists of 3 treatments.
While the subplots were fertilized with the LEISA technique which consisted of 6 treatments so that 18 treatment
combinations were obtained with 3 replications. Observations were made on plant height, number of leaves, stem
diameter, number of roots, wet weight and dry weight of shoot. Observational data were analyzed using analysis
of variance, followed by the DMRT α0.05 if there was a significant effect. The results showed that seed
biomatriconditioning treatment with rhizobacteria integrated with the LEISA technique was able to increase the
growth of areca nut seedlings. The integration between L1R biomatriconditioning and the application of organic
plus fertilizer + 100% inorganic fertilizer showed a better growth performance of areca nut on plant height,
number of leaves, stem diameter, number of roots, wet weight and dry weight of betel nut which were significantly
different with control and application 100% inorganic fertilizer, but not significantly different from organic plus
fertilizer, organic plus fertilizer + 50% inorganic fertilizer and organic plus fertilizer + 25% inorganic fertilizer.
As a conclusion, pre-planting seed treatment with L1R biomatriconditioning is very important to increase areca
nut seed germination. To increase the growth of areca nut seedlings, further fertilization needs to be done with
organic plus fertilizer or a combination of organic plus fertilizer + 25% inorganic fertilizer.


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Cox, S. M. Ullah and H. Zoellner, 2016. Oral and systemic health effects of compulsive areca nut use

neuropathology of drug addictions and substance misuse. Academic Press. 2016, pp. 785-793.

Sari, L.M., G. Permana and E. Ibrahim, 2017. Potential antioxidant and cytotoxic activities of areca nut (Areca

catechu Linn.) extract in human oral squamous cell carcinoma and keratinocyte cells. Asian Journal of

Pharmaceutical and Clinical Research, 10: 286-291.

Lazim, Y., S.M. Salit, E.S. Zainudin, M. Mustapha and M. Jawaid, 2014. Effect of alkali treatment on the

physical, mechanical, and morphological properties of waste betel nut (Areca catechu) husk fibre.

BioResources. 9: 7721–7736.

Setyawan, H.Y., Sunaryo, B. Waluyo, Merlya, A. Choirun, A. Nizori, and Sahrial, 2020. Energy potential from

areca palm through direct combustion and pyrolysis in Indonesia: a review. Indonesian Food Science and

Technology Journal. 4: 19-26.

Sutariati, G.A.K., A. Khaeruni, Y.B. Pasolon and L. Mudi. 2016.

The effect of seed bio-invigoration using indigenous rhizobacteria to improve viability and vigor of upland rice

(oryza sativa L.) Seeds. International Journal of PharmTech Research, 9: 565-573.

Sutariati, G.A.K., Muhidin, T.C. Rakian, L.O. AFa, I. Wiadanta, L. Mudi, G.R. adimantara and S. Leomo,

The effect of integrated application of pre-plant seed bio-invigoration, organic and inorganic fertilizer on

the growth and yield of local upland rice. Bioscience Research, 15: 160-165.

Wangadi, S., G.A.K. Sutariati dan A. Khaeruni, 2018. Kajian teknik bio-invigorasi benih dan LEISA untuk

meningkatkan pertumbuhan padi gogo lokal pada skala rumah kasa. J. Berkala Penelitian Agronomi 6: 1-10.

Nuraini, A., Y. Yuwariah dan Y. Rochayat, 2015. Pengembangan produksi pertanian lahan kering dengan

sistem low external input sustainable agriculture (LEISA) di Desa Cigadog, dan Mandalagiri Kecamatan,

Leuwisari Kabupaten Tasikmalaya. Dharmakarya: Jurnal Aplikasi Ipteks untuk Masyarakat, 4: 113 – 118.

Oberč, B.P. & A.A. Schnell, 2020. Approaches to sustainable agriculture. Exploring the pathways towards the

future of farming. Brussels, Belgium: IUCN EURO.

Sridhar, S.M. & C.P. Swaminathan, 2020. Low External Inputs In Sustainable Agriculture (LEISA).

Conference: Academic PG credit seminar. DOI:10.13140/RG.2.2.10373.58081.

Prihastuti, 2016. The strategy of developing sweet potato cultivation in tidal land. El-Hayah. 6. 01. 1-6. DOI:


Setiyo, Y.H., B. Gunam, I. Gunadnya, I.D.P. Yulianti, N.I. Triani, 2021. Application of the LEISA system to

improve soil properties and increase potato productivity. International Journal of Pharmaceutical Research,

: 3014-3021. DOI:

Islam, M.F., G. Akter, A. Hossain, M.N. Dilip, 2017. Effect of Organic, Inorganic Fertilizers and Plant

Spacing on the Growth and Yield of Cabbage. Agriculture. 7(31). DOI:10.3390/agriculture7040031.

Sutrisno, S. & E. Yusnawan, 2018. Effect of manure and inorganic fertilizers on vegetative, generative

characteristics, nutrient, and secondary metabolite con-tents of mungbean. Biosaintifika: Journal of Biology &

Biology Education, 10: 56-65.

Anggraheni, Y.G.D., F. Nuro, and Y.B. Paradisa, 2019. Effect of organic fertilizer on growth and yield of chili

pepper. The 3rd Satreps Conference 2018. The 376th RISH Symposium and The 9th Flagship Symposium of

Tropical Plant Biomass. Vol 1, Mei 2019. Bogor November 22nd, 2018.

International Journal of Science, Technology & Management ISSN: 2722 - 4015

Sutariati, G.A.K., N.M. Rahni, A. Madiki, L. Mudi, I.M. Guyasa and Zahrima, 2020. Characterization of

endophytic-rhizobacteria from areca nut rhizosphere to dissolve phosphates, nitrogen fixation of IAA hormone

synthesis. Pak. J. Biol. Sci., 23: 240-247.

Li, Z., C. Song and Y. Yi, 2020. Characterization of plant growth-promoting rhizobacteria from perennial

ryegrass and genome mining of novel antimicrobial gene clusters. BMC Genomics, 21(2020) 157.

Mei, C., R.L. Chretien, B.S. Amaradasa, Y. He, A. Turner and S. Lowman, 2021. Characterization of

phosphate solubilizing bacterial endophytes and plant growth promotion in vitro and in greenhouse.

Microorganisms, 9(2021) 1935.

Zafar, S., A. Bano and T.U.l. Hassan, Evaluation of indole-3-acetic acid deficient mutants of Pseudomonas

moraviensis and its role in mitigation of salt stress in Cicer arietinum L. Pak. J. Bot., 54: 1-10. DOI:

Sutariati, G.A.K., N.M. Rahni, L. Mudi, R.R. Maharani and G.N.A. Wibawa, 2021.

The effectiveness of endo-rhizo bacterial isolated from areca nut rizosphere (Areca cathecu L.) in breaking

dormancy and improvement of seed vigor. IOP Conference Series: Earth and Environmental Science, 807(4):


Timmusk, S., 2003. Mechanism of actions of the the plant-growth-promoting rhizo bacterium Paenibacillus

polymixa [Dissertation]. Uppsala, Sweden: Departement of Cell and Molecular Biology, Uppsala University.

Sutariati, G.A.K., Widodo, Sudarsono dan S. Ilyas, 2006. Pengaruh perlakuan plant growth promoting

rhizobacteria terhadap pertumbuhan bibit tanaman padi. Buletin Agronomi, 34: 46-54.

Sutariati, G.A.K., Widodo, Sudarsono, dan S. Ilyas, 2006. Efektivitas agens biokontrol untuk meningkatkan

pertumbuhan dan hasil cabai serta mengendalikan penyakit antraknosa di rumah kaca. Agriplus, 16: 103-111.

Sutariati, G.A.K. dan L.O. Safuan, 2012. Perlakuan benih dengan rizobakteri meningkatkan mutu benih dan

hasil cabai (Capsicum annuum L.). J. Agron. Indonesia 40: 125-131.

Sutariati, G.A.K. dan A. Wahab, 2011. Karakter fisiologi dan kemangkusan rizobakteri indigenos sulawesi

tenggara sebagai pemacu pertumbuhan tanaman cabai. J. Hort. 22: 57-64.

Ilyas, S., G.A.K. Sutariati, F.C. Suwarno and Sudarsono, 2002. Matriconditioning improved quality and

protein level of medium vigor hot pepper seed. Seed Technol., 24: 65-75.

Silva, H.S.A., R.S.R. Romeiro, D. Macagnan, B.A.H. Vieira, M.C.B. Pereira and A. Mounteer, 2004.

Rhizobacterial induction of systemic resistance in tomato plants: non-specific protection and increase in

enzyme activities. Biol Control, 29:288-295.

Yan, Z., C.M. Ryu, J. McInroy, M.S. Reddy, F. Woods, M. Wilson and J.W. Koepper. 2004. Induction of

systemic resistance against tomato late blight by plant growth promoting rhizobacteria (PGPR).

Zhang, Y., 2004. Biocontrol of sclerotia stem root of canola by bacterial antagonist and study of biocntrol

mechanism involved. Thesis. Departemen of Plant Science, University of Manitoba. Canada.

Wahid A, A. Noreen, M.A. Shahzad, Basra, S. Gelani and M. Farooq, 2008. Priming-induced metabolic

changes in sunflower (Helianthus annuus) achenes improve germination and seedling growth. Botanical

Studies, 49: 343-350.

Desai BB, Kotecha PM, Salunkhe DK. 1997. Seeds handbook, biology, production, processing and storage.

Marcel Dekker Inc. USA.

Moradi A. and Younesi O. 2009. Effects of osmo- and hydro-priming on seed parameters of grain sorghum

(Sorghum Bicolor L.). Australian Journal Of Basic And Applied Sciences, 3: 1696-1700

Sutariati, G.A.K. dan A. Wahab. 2010. Isolasi dan uji kemampuan rizobakteri indigenous sebagai agens

pengendali hayati penyakit pada tanaman cabai. Jurnal Hortikultura, 20: 86-95.

Chaves, E.I.D., V.F. Guimarães, E.C.G. Vendruscolo, M. Fonseca dos Santos, F. Freitas de Oliveira, J.A.

Cordeiro de Abreu, M.P. Camargo, V.S. Schneider, E. Maltempi de Souza, L.M. Cruz and E. Soares de

Vasconcelos. 2019. Interactions between endophytic bacteria and their effects on poaceae growth performance

in different inoculation and fertilization conditions. AJCS, 13: 69-79.

Liu, Z., X. Zhang and L. Li, 2021. Isolation and characterization of three plant growth-promoting rhizobacteria

for growth enhancement of rice seedling. J Plant Growth Regul. (2021).

Ashrafuzzaman, M., F.A. Hossen, M.R. Ismail, Md.A. Hoque, M.Z. Islam, S.M. Shahidullah and S. Meon.

Efficiency of plant growth-promoting rhizobacteria (PGPR) for the enhancement of rice growth. African

Journal of Biotechnology., 8:1247-1252.

International Journal of Science, Technology & Management ISSN: 2722 - 4015

ElSorra, E.I., J. I Domingo, T. Manuel and B. Rainer, 2007. Tryptophan-dependent productionof indole-3-

acetic acid (IAA) affects level of plant growth promotion by Bacillus amyloliquefaciens FZB42. The American

Phytopathological Society., MPMI 20: 619–626.

Park, K.H., C.Y. Lee and H.J. Son, 2009. Mechanism of insoluble phosphate solubilization by Pseudomonas

fluorescens RAF15 isolated from gingseng rhizophre and its plant growth-promoting activities. Lett. Appl.

Microbiol, 49: 222-8.

Mehrab, Y.H., A. Rahmani, G. Noormohammadi and A. Ayneband. 2010. Plant growth promoting

rhizobacteria increase growth, yield and nitrogen fixation in Phaseolus vulgaris. Journal of Plant Nutrition,

: 1733- 1743.

Sutariati, G.A.K dan A. Wahab, 2012. Kararkter fisiologis dan kemangkusan rizobakteri indigenus sebagai

pemacu pertumbuhan Tanaman Cabai. J. Hotikultura, 22: 57-64.

Datta, M., R. Palit, C. Sengupta, M.K. Pandit and S. Banerjee, 2011. Plant growth promoting rhizobacteria

enhance growth and yield of chilli (Capsicum annuum L.) under field conditions. Australian Journal of Crop

Sciences 5: 531-36.

Vacheron, J., G. Desbrosses, M.L. Bouffaud, B. Touraine, Y. Moënne-Loccoz, D. Muller, L. Legendre, F.

Wisniewski-Dyé and C. Prigent-Combaret, 2013. Plant growth-promoting rhizobacteria and root system

functioning. Front Plant Sci. 4: 1-19.

Elango. R, R. Parthasarathi and S. Megala, 2013. Field level studies on the association of plant growth

promoting rhizobacteria (PGPR) in Gloriosa Superba L. rhizosphere. Indian Streams Research Journal, 3:1-

Ilyas, S., K.V. Asie and G.A.K. Sutariati, 2014. Biomatriconditioning or biopriming with biofungicides or

biological agents applied on hot pepper (Capsicum annuum L.) seeds reduced seedborne Colletotrichum

capsici and increased seed quality and yield ISHS Acta Hortic. 1105: 89–96.

Ambawade, S. and G.R. Pathade, 2015. Production of gibberellic acid by Bacillus siamensis BE 76 isolated

from banana plant (Musa spp.). International Journal of Science and Research, 4: 2319–7064.

Sutariati, G.A.K., K. Jusoff, I.G.R. Sadimantara, A. Khaeruni, Muhidin and Meisanti, 2013. Effectiveness of

bio-invigoration technologies on seed viability and vigor of cocoa (Theobroma cacao L.). World Applied

Sciences Journal (Natural Resources Research and Development in Sulawesi Indonesia), 26: 31-36.

Mujahid, T.Y., S.A. Subhan, A. Wahab, J. Masnoon, N. Ahmed and T. Abbas, 2015 Effects of different

physical and chemical parameters on phosphate solubilization activity of plant growth promoting bacteria

isolated from indigenous soil Journal of Pharmacy and Nutrition Sciences, 5: 64-70.

Zahid, M., M.K. Abbasi, S. Hameed and N. Rahim, 2015. Isolation and identification of indigenous plant

growth promoting rhizobacteria from Himalayan region of Kashmir and their effect on improving growth and

nutrient contents of maize (Zea mays L.). Front Microbiol. 6: 1-10.

Widayanti, T., 2007. Isolasi dan karakterisasi Bacillus sp. indigenus penghasil asam indol asetat asal tanah

rizosfer. Skripsi. Depatermen Biologi, Fakultas Metematika dan Ilmu Pengetahuan Alam. Institut Pertanian


Sutariati, G.A.K., 2006. Perlakuan benih dengan agens biokontrol untuk pengendalian penyakit antraknosa,

peningkatan hasil dan mutu benih padi. Disertasi. Program Pascasarjana Institut Pertanian Bogor.

Mishra, R.K., O. Prakash, M. Alam and A. Dikshit, 2010. Influence of plant growth promoting rhizobacteria

(PGPR) on the productivity of Pelargonium graveolens L. Herit. Recent Research in Science and Technology,

: 53-57.

Erturk, Y., S. Ercisli., A. Haznedar dan R. Cakmakci, 2010. Effects of plant growth promoting rhizobacteria

(PGPR) on rooting and root growth of kiwifruit (Actinidia deliciosa) stem cuttings. Biol. Res., 43: 91-98.

Chauhan, H., D. Bagyaraj, G. Selvakumar and S. Sundaram, 2015. Novel plant growth promoting

rhizobacteria-prospects and potential. Appl. Soil Ecol. 95: 38–53. doi: 10.1016/j.apsoil.2015.05.011.

Sutariati, G.A.K., N.M. Rahni, A. Madiki, and I.M. Guyasa. 2020. Characterization of Endophyticrhizobacteria from areca nut rhizosphere to dissolve phosphates, nitrogen fixation of IAA hormone synthesis.

Pakistan Journal Of Biological Sciences: PJBS, 23: 240-247.

Bhutani, N., R. Maheshwari, N. Monika and S.D. Pooja, 2018. Optimization of IAA production by endophytic

Bacillus spp. from Vigna radiata for their potential use as plant growth promoters. Israel Journal of Plant

Sciences, 65. 10.1163/22238980-00001025.

Andriūnaitė, E., I. Tamošiūnė, M. Aleksandravičiūtė, D. Gelvonauskienė, J. Vinskienė, R. Rugienius and D.

Baniulis, 2021. Stimulation of Nicotiana tabacum L. in vitro shoot growth by endophytic Bacillus cereus

group bacteria. Microorganisms, 9: 1893.

How to Cite
Gusti Ayu Kade Sutariati, Muhidin, Nini Mila Rahni, Gusti Ngurah Adi Wibawa, & La Mudi. (2021). The Effectiveness Of Pre-Planting Seeds Bio-Matriconditioning And Application Of Fertilization With Leisa Technique On Growth Of Areca Nut Seedling (Areca Catechu L.). International Journal of Science, Technology & Management, 2(6), 2053-2060.